In vitro mutagenesis of pruacan (Pimpinella pruatjan Molk): effect of chemical mutagen EMS and lethal dose determination

Pruacan is a rare medicinal plant endemic to the Dieng Plateau of Central Java, Indonesia which has properties as an aphrodisiac. Increasing genetic variability ofthis in order support breeding programs and increase productivity active ingredients this important. This study aimed evaluate effect chemical mutagen EMS on growth development vitro culture pruacan lethal dose determination. Embryogenic callus were soaked 0, 0.1, 0.3, 0.5 0.7% for 2 hours then cultured regeneration medium containing DKW basal salt + IBA 5 mg/l. suppressed somatic embryos culture. The lowest explant fresh weight, percentage explants forming plantlets number per obtained from 0.7 % (2.38 g, 91.67 6.22, respectively). Lethal 50 was 0.61, 0.645 0.53% based plantlets. And explant, respectively. can be applied induce mutation pruacan.